Table 2.
Features of pETBTY825 ORFs
| ORF | Position (bp) |
Strand | Gene | Length (aa)a | Translation signalb | Source | Description | Identity (%) | Overlapc (aa) | Accession no. | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Start | Stop | ||||||||||
| 1 | 231 | 455 | + | repA | 74 | GAGGTTTTTATTATG | S. aureus(pETB) | pETB_p18 (replication initiator protein A) | 100 | 74/74 | BAB78416 |
| 2 | 642 | 785 | + | rep | 47 | GAGAATAATGATATG | S. aureus TCH130 | Hypothetical protein (truncated replication protein) | 60.6 | 33/47 | ZP_04868980 |
| 3 | 1002 | 1217 | + | 71 | AGGGCTATGTAAAGAATTG | S. aureus(pETB) | pETB_p19 (transcriptional regulator protein) | 100 | 71/71 | NP_478362 | |
| 4 | 1590 | 3164 | + | repR | 524 | AGGAGGTGCAGACAATG | S. aureus(pETB) | pETB_p20 (plasmid replication protein RepR) | 100 | 524/524 | NP_478363 |
| 5 | 4397 | 4564 | + | 55 | S. aureus(pETB) | pETB_p22 (lipase) | 100 | 55/55 | NP_478365 | ||
| 6 | 4647 | 5378 | + | 243 | GAGGTATTCTTAATAAAATG | S. aureus(pETB) | pETB_p23 (cell wall-associated biofilm protein) | 92.4 | 243/243 | NP_478366 | |
| 7 | 5738 | 7510 | − | abiK | 590 | AGGAGAAAGGCTATG | S. aureus(pETB) | Abortive infection protein K | 100 | 590/590 | NP_478367 |
| 8 | 7914 | 8261 | − | cadX | 115 | AGGGTGCGATTTTATATG | S. lugdunensis(pLUG) | CadX | 100 | 115/115 | NP_054018 |
| 9 | 8280 | 8897 | − | cadD | 205 | GAGGTGTAATTATG | S. aureus(pETB) | Cadmium-binding protein | 99.5 | 205/205 | NP_478377 |
| 10 | 8966 | 9109 | − | 48 | S. epidernidis BCM-HMP0060 | Hypothetical protein | 97.9 | 48/48 | ZP_04824204 | ||
| 11 | 9670 | 9858 | − | 62 | AGGATTATATCGAAAACGTATG | S. epidernidis BCM-HMP0060 | Replication protein Rep | 93.4 | 62/62 | ZP_04824202 | |
| 12 | 9989 | 10960 | − | 323 | AGAGGTTTTTGTATG | S. saprophyticus ATCC 15305 | Replication initiator protein | 99.4 | 323/323 | YP_302585 | |
| 13 | 11475 | 12167 | + | 230 | GGAGGCCATTATATG | S. epidernidis BCM-HMP0060 | Partitioning protein | 80.9 | 230/230 | ZP_04824200 | |
| 14 | 12788 | 13558 | − | smpA | 256 | AGGAGGATCAATCGTAAAATG | S. epidernidis 968 | ABC transporter membrane protein | 100 | 256/256 | CAA83062 |
| 15 | 13560 | 14255 | − | stpA | 231 | AGGAGATAATTGTATG | S. epidernidis W23144 | ABC transporter ATP-binding protein | 100 | 231/231 | ZP_04796098 |
| 16 | 14792 | 16258 | + | msrA | 488 | AGGAGTGTATAAATATG | S. epidernidis W23144 | ABC transpoter permease protein (erythromycin resistance protein, MsrA) | 100 | 488/488 | ZP_04796097 |
| 17 | 16482 | 16910 | − | Sin | 142 | GGAGATCGATTCGTTGTG | S. aureus USA300_TCH959 | Recombinase Sin | 97.9 | 142/142 | YP_001569089 |
| 18 | 17217 | 17795 | − | binR | 192 | AGGAGGTTTGTATTTTG | S. aureus CF-Marseille | Tn552 DNA invertase BinR | 98.9 | 192/192 | ZP_04839235 |
| 19 | 18059 | 18439 | − | blaI | 126 | S. epidernidis ATCC 12228 | Beta-lactamase repressor BlaI | 100 | 126/126 | NP_863211 | |
| 20 | 18429 | 20228 | − | blaR1 | 599 | S. aureus JKD6008 | Beta-lactamase regulatory protein BlaR1 | 100 | 585/599 | ZP_03563212 | |
| 21 | 20293 | 21138 | + | blaZ | 281 | GGAGGGTTTATTTTG | S. aureus MRSA252 | Beta-lactamase | 99.6 | 281/281 | NP_878023 |
| 22 | 21500 | 21685 | − | 61 | AGGTTATGAAAGTAAATGTATG | S. epidernidis RP62A | Conserved hypothetical protein | 95.1 | 61/61 | YP_189789 | |
| 23 | 21757 | 21960 | − | 67 | AGGGGGAGTATCTTTG | S. epidernidis RP62A | Conserved hypothetical protein | 95.7 | 47/67 | YP_189789 | |
| 24 | 22751 | 23482 | − | 243 | GGAGGTAAGTTTTG | S. epidernidis RP62A | Conserved hypothetical protein | 97.1 | 243/243 | YP_189787 | |
| 25 | 23788 | 24666 | − | 292 | AGGACTGTTATATG | S. epidernidis ATCC 12228 | Hypothetical protein | 93.2 | 281/292 | NP_863227 | |
| 26 | 24729 | 25901 | + | IS256 | 390 | AGGAGGACTTTTACATG | E. faecalis V583 | IS256 transposase | 100 | 390/390 | NP_813928 |
| 27 | 26041 | 27480 | − | aac(6′)-aph(2′) | 479 | AGGTGATAAATAAATG | S. aureus Mu50 | Bifunctional AAC(6′)/APH(2″):6′-aminoglycoside N-acetyltransferase and 2″-aminoglycoside phosphotransferase | 100 | 479/479 | NP_115315 |
| 28 | 27481 | 27885 | − | 134 | AGGAGTCTGGACTTG | S. aureus(pLW043) | Acetyltransferase GNAT family protein | 100 | 134/134 | NP_878007 | |
| 29 | 27930 | 29102 | − | IS256 | 390 | AGGAGGACTTTTACATG | E. faecalis V583 | IS256 transposase | 100 | 390/390 | NP_813928 |
| 30 | 29203 | 30294 | − | traA | 363 | AGAGGAGGTAAAATCATG | S. epidernidis W23144 | Nickase TraA | 100 | 363/363 | ZP_03986061 |
| 31 | 30498 | 30767 | + | 89 | GGAGTTTTTTAATG | S. epidernidis W23144 | Conserved hypothetical protein | 100 | 89/89 | ZP_03986060 | |
| 32 | 30784 | 31023 | + | 79 | S. epidernidis W23144 | Conserved hypothetical protein | 100 | 79/79 | ZP_03986059 | ||
| 33 | 31131 | 31805 | − | IS257 | 224 | AGGAGTCTTCTGTATG | S. aureus(pV030-8) | IS257 transposase | 98.7 | 224/224 | YP_001653101 |
| 34 | 32145 | 32966 | + | 273 | AGGAGACCTAGTTAATG | S. aureus MRSA252 | LysR family regulatory protein | 96.3 | 273/273 | YP_040145 | |
| 35 | 33683 | 33823 | − | 46 | S. aureus(pEDINA) | pEDINA_p50 (transcriptional regulator) | 97.8 | 45/46 | YP_001573922 | ||
| 36 | 34209 | 34736 | + | IS257 | 175 | S. aureus(pETB) | pETB_p37 (IS257 transposase) | 100 | 175/175 | NP_478380 | |
| 37 | 34771 | 35913 | − | 380 | AGGAGAAACTATG | S. aureus(pETB) | pETB_p38 (putative ATP/GTP-binding protein) | 99.7 | 380/380 | NP_478381 | |
| 38 | 36042 | 36272 | + | 76 | TAAGCTGCTGCTGTATATTATG | S. aureus(pETB) | pETB_p39 (conserved hypothetical protein) | 100 | 76/76 | NP_478382 | |
| 39 | 36635 | 36823 | + | sacaA | 62 | TAAAGCGTGGTGATTCTTATG | S. aureus(pETB) | pETB_p40 (lantibiotic structural protein Sac-alpha-A) | 100 | 62/62 | NP_478383 |
| 40 | 36847 | 37050 | + | sacbA | 67 | TAAGGTGGTATTTTTATG | S. aureus(pETB) | pETB_p41 (lantibiotic structural protein Sac-beta-A) | 100 | 67/67 | NP_478384 |
| 41 | 37069 | 39966 | + | sacM1 | 965 | GGAGATAGTTCATAATG | S. aureus(pETB) | pETB_p42 (lantibiotic mersacidin modifying enzyme SacM1) | 100 | 965/965 | NP_478385 |
| 42 | 39968 | 42130 | + | sacT | 720 | GAGGTGTAATATG | S. aureus(pETB) | pETB_p43 (lantibiotic mersacidin ABC transporter system SacT) | 100 | 720/720 | NP_478386 |
| 43 | 42127 | 44880 | + | sacM2 | 917 | AAGGAGTGTGGAGTTTG | S. aureus(pETB) | pETB_p44 (lantibiotic mersacidin modifying enzyme SacM2) | 99.9 | 917/917 | NP_478387 |
| 44 | 44896 | 45996 | + | 366 | AGGAGCGTAAATATTTG | S. aureus(pETB) | pETB_p45 (conserved hypothetical protein) | 100 | 365/366 | NP_478388 | |
| 45 | 46011 | 46880 | + | 289 | AGGAGAATTCTGATG | S. aureus(pETB) | pETB_p46 (multidrug efflux ABC transporter ATP-binding protein) | 100 | 289/289 | NP_478389 | |
| 46 | 46864 | 47589 | + | 241 | GGAGGTTCTAAAATTG | S. aureus(pETB) | pETB_p47 (putative membrane protein) | 100 | 241/241 | NP_478390 | |
| 47 | 47618 | 47791 | + | 57 | GGAGGAATTTTAATG | S. aureus(pETB) | pETB_p48 (conserved hypothetical protein) | 100 | 57/57 | NP_478391 | |
| 48 | 48118 | 48792 | − | IS257 | 224 | GAGGTGCAGAGGATG | S. aureus(pETB) | pETB_p49 (IS257 transposase) | 100 | 224/224 | NP_478392 |
| 49 | 49007 | 49573 | − | res | 188 | GAGGTTATATTTGAATG | S. aureus(pETB) | pETB_p50 (recombinase Res) | 100 | 188/188 | NP_478393 |
| 50 | 50164 | 50955 | + | 263 | AGGTACCAATTTATG | S. aureus(pETB) | pETB_p01 (replication-associated protein) | 100 | 263/263 | NP_478344 | |
| 51 | 51488 | 52231 | − | ednC | 247 | AAGGAGTCTTTTATG | S. aureus(pETB) | epidermal cell differentiation inhibitor EDINC | 100 | 247/247 | NP_478345 |
| 52 | 52801 | 53631 | − | 276 | AAGGAGAATGAGGCATTG | S. aureus(pETB) | pETB_p03 (conserved hypothetical protein) | 99.6 | 276/276 | NP_478346 | |
| 53 | 53708 | 54022 | − | 104 | AAGGAGAGAAAATAATG | S. aureus(pETB) | pETB_p04 (conserved hypothetical protein) | 100 | 104/104 | NP_478347 | |
| 54 | 54175 | 54591 | + | 138 | GAGGTGTATTAAAATG | S. aureus(pETB) | pETB_p05 (conserved hypothetical protein) | 100 | 138/138 | NP_478348 | |
| 55 | 54833 | 55666 | + | etb | 277 | AAGGAGGTTTTATATATG | S. aureus(pETB) | exfoliative toxin B | 100 | 277/277 | NP_478350 |
| 56 | 55760 | 55921 | − | 53 | S. aureus MN8 | conserved hypothetical protein | 56.9 | 51/53 | ZP_03987549 | ||
| 57 | 56732 | 56881 | − | 49 | AGGAGGCATTTATTATG | S. aureus(pETB) | pETB_p11 (conserved hypothetical protein) | 100 | 49/49 | NP_478354 | |
| 58 | 57008 | 57958 | − | 316 | AAGGAGTAGTTAAGATG | S. aureus(pETB) | pETB_p12 (extracellular protein) | 100 | 316/316 | NP_478355 | |
| 59 | 58022 | 58234 | − | 70 | GGAGGTAACCTAAATATG | S. aureus(pETB) | pETB_p13 (conserved hypothetical protein) | 100 | 70/70 | NP_478356 | |
| 60 | 58309 | 58653 | − | mutS | 114 | GGAACAATTG | S. aureus(pWBG749) | putative DNA mismatch repair protein MutS | 83.1 | 98/118 | NP_478357 |
| 61 | 58713 | 58925 | − | 70 | GAGGGTTTTACAAATG | S. aureus(pETB) | pETB_p15 (conserved hypothetical protein) | 100 | 70/70 | NP_478358 | |
| 62 | 59090 | 59332 | − | 80 | AGGAGAGATACTATG | S. aureus(pETB) | pETB_p16 (conserved hypothetical protein) | 100 | 80/80 | NP_478359 | |
| 63 | 59501 | 60307 | − | parA | 268 | GGAGGTGGAAGCAATG | S. aureus(pETB) | pETB_p17 (plasmid partition protein ParA) | 100 | 268/268 | NP_478360 |
a
aa, amino acids.
b
Underlining indicates a putative ribosome binding site complementary to the 3′ end of the 16S rRNA; boldface indicates the start codon.
c
Overlap indicates the number of overlapping amino acids/total number of amino acids.