Fig 2.

Clonal frequency of single-substitution resistance-associated mutations in genotype 1a-infected patients at day 4 dosed at ≥3 mg of GS-5885. TOPO TA cloning and subsequent sequencing of inserts were performed from amplicons used in population-sequencing reactions. “Wildtype” indicates no mutations at NS5A positions 24, 25, 28, 30, 31, 58, 62, and 93 compared to the 1a-H77 reference strain. K24E, K24N, and K24R mutations were combined into one category. For all graphs, n = number of patients in the dosing cohort from which clonal sequences were derived.