Table 3.

Phenotypic analysis of subject isolates

GT and subject	Baseline RAM	Clonal NS5A variant(s) at day 4 (frequency)a	GS-5885 EC50 (nM)b			Daclatasvir EC50 (nM)b
			Baseline	Day 4	Fold change	Baseline	Day 4	Fold change
1a
O		WT (10%), M28T (3%), Q30H (23%), Q30R (53%), L31M (3%), Y93C (7%)	0.002	0.071	35.5	0.008	>1.5	>196
I		WT (44%), Q30R (31%), L31M (25%)	0.005	0.403	88	0.007	2.07	292
BJ		Q30H (3%), Q30R (67%), Q30E (3%), H58D (3%), Y93C (7%), Y93H (7%), Q30R+Y93C (10%)	0.017	4.3	355.6	0.003	0.8	267
L		WT (13%), M28T (7%), Q30H (7%), Q30R (7%), L31M (33%), Y93C (3%), Q30R+Y93C (10%)	0.004	>1.5	>375	0.005	>1.5	>288
BR		Q30R (86%), L31M (4%), H58D (11%)	0.009	3.5	389	0.007	2.1	300
T		WT (3%), Q30R (40%), L31M (3%), H58D (47%), Q30R+H58D (7%)	0.006	0.52	87	0.003	0.49	163
BI		Q30R (87%), Q30E (3%), K24R+Q30R (10%)	0.006	>50	>8,333	0.006	22.2	3700
BK		Q30K (7%), Q30R (66%), Y93C (14%), Y93H (7%), Q30R+Y93H (3%), Q30R+Y93C (3%)	0.004	>50	>12,500	0.004	>50	>12,500
Q	12% Y93C	M28T (3%), Q30R (26%), L31M (7%), Y93C (52%), M28V+Q30R (3%), M28T+Y93C (7%), Q30R+Y93C (3%)	0.014	0.285	20.4	0.057	0.487	8.5
1b
AR		Y93Hc	0.001	0.994	994	0.013	0.12	9.6

^aClonal variants were determined by deep sequencing.

^bFold change in the EC₅₀ was calculated by dividing the day 4 EC₅₀ by the baseline EC₅₀.

^cGenotype 1b sample, day 14 population sequencing EC₅₀.