Fig 5.

Deletion of the 1st alpha helix is sufficient to reproduce and to exacerbate the multinucleate phenotype of abiA-null cells. Cells were cultured for the time taken for 10 divisions on glass coverslips before fixation and stained with Texas Red phalloidin and DAPI. The number and extent of multinucleate cells were then counted. (a) Examples of normal and multinucleate cells found in abiA-null cells expressing WT Abi, ΔNt Abi, ΔAbiΔ, and the HSPC300-GFP vector only (Null). (b) Percentages of cells with 1, 2, 3, 4, or ≥5 nuclei cells rescued with the different Abi constructs. WT Abi, full-length Abi; ΔNt, N-terminally truncated Abi; ΔCt₂, C-terminally truncated Abi; ΔAbiΔ, combination of both N-terminally and C-terminally truncated Abi; Null, HSPC300-GFP-only vector.