Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| S. meliloti strains | ||
| Rm1021 | Wild type (Smr) | 46 |
| Rm7017 | exoD17::Tn5 (Smr Nmr) | 17 |
| Rm7031 | exoA31::Tn5 (Smr Nmr) | 17 |
| Rm7210 | exoY210::Tn5 (Smr Nmr) | 17 |
| Rm7210Sp | exoY210::Tn5-233 (Smr Spr Gmr) | This study |
| Rm8332 | exoQ332::Tn5 (Smr Spr) | 47 |
| Rm8530 | expR101 (Smr) | 48 |
| Rm8532 | expR101 exoA31::Tn5 (Smr Spr) | 48 |
| MB801 | exoX::Tn5 (Smr Nmr) | M. Barnett |
| APL41 | katA::uidA pAPL10 integrated into Rm1021 | This study |
| APL42 | katA::uidA pAPL10 integrated into Rm7210 | This study |
| APL43 | katA::uidA pAPL10 integrated into MB801 | This study |
| Plasmids | ||
| pRK607 | pRK2013Ω::Tn5-233 (Spr Gmr) | 29 |
| pRF771 | Ptrp overexpression vector, pTE3 with extended polylinker (Tcr) | 49 |
| pE65 | nodD3 in pTE3 (Tcr) | 50 |
| pMB89 | syrA in pTE3 (Tcr) | 51 |
| pS73 | syrM in pTE3 (Tcr) | 32 |
| pDW33 | Terminator and polylinker preceding uidA (Apr Hyr) | 52 |
| pAPL10 | PkatA in pDW33 | This study |