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In vivo dimerization of SbmA. (A) Bacterial two-hybrid assay. E. coli BTH101 cells were cotransformed with pUT18C-zip and pKT25-zip vectors as a positive control (1), pKNT25-sbma and empty pUT18 (2), and pUT18-sbma and pKNT25-sbma (3). (B) β-Galactosidase assay to measure the enzymatic activity expressed as Miller units (M.U.). Bar numbers correspond to plate numbers in panel A. Results are the means of four independent experiments.
