Fig 2.

Hydrolysis of α-1→2 and α-1→3 mannobioses by P. gingivalis W50 and single-isogenic mutant strains analyzed by TLC on Keiselgel F₂₅₄ HPTLC plates in n-butanol–ethanol–water (5:5:3, by volume). The experiments were performed with P. gingivalis W50, ΔPG0032, ΔPG0902, ΔPG0973, ΔPG1711, and ΔPG1712 as described in the legend to Fig. 1. (A) α-1→2 mannobiose. (B) α-1→3 mannobiose. Lanes: 1, mannobiose standard; 2, mannose standard; 3, W50, time 0; 4, W50, 21 h; 5, ΔPG0032, 21 h; 6, ΔPG0902, 21 h; 7, ΔPG1711, 21 h; 8, ΔPG1712, 21 h; 9, ΔPG0973, 21 h.