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Hydrolysis of 4-nitrophenyl α-d-mannopyranoside and 4-nitrophenyl β-d-mannopyranoside by P. gingivalis whole-cell suspensions
| pH | Rate of hydrolysis (nmol 4-NP produced/h/OD600 unit of cells)a |
|
|---|---|---|
| α-Mannosidase activity | β-Mannosidase activity | |
| 4.5 | ∼0 | ∼0 |
| 5.0 | 0.0224 | 1.519 |
| 5.5 | 0.156 | 1.959 |
| 6.0 | 0.270 | 2.171 |
| 6.5 | 0.227 | 2.154 |
| 7.0 | 0.120 | 0.681 |
| 7.5 | 0.097 | 0.167 |
Rates of hydrolysis were measured in either sodium acetate buffers or Tris-HCl buffers containing 4 mM Ca2+ at 37°C.
