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Electrophoretic mobility shift assays. (A) More pgdA transcript (50 nM) bound to apo-AcnB with increasing protein concentration. (B) Addition of iron and reductant abolished binding, whereas the iron chelator dipyridyl promoted binding. DTT, dithiothreitol. (C) Radiolabeled pgdA probe was outcompeted by specific unlabeled competitor RNA at 100× molar excess. (D) Use of nonspecific protein bovine serum albumin (3,000 nM) instead of apo-AcnB did not result in a shift.
