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. 2013 Dec;195(23):5381–5390. doi: 10.1128/JB.02165-12

Fig 2.

Fig 2

Integrated (A) and circular (B) forms of ICESluvan in transconjugants E. faecium MM5-F9a, E. faecalis OG5-F9a, and E. faecalis JH5-F9a, as well as donor S. lutetiensis 5-F9. The left and right ends of ICESluvan are colored gray and black, respectively, while the native chromosomal DNA is shown by hatching. The sequences of the 20-bp ends of ICESluvan are also shown. Black and gray arrows indicate the positions and directions of primers for the left and right end of ICESluvan, respectively. The positions of primers designed from native E. faecium MM5-F9a regions are indicated with black-and-white arrows. PCR and sequencing confirmed both the integrated and the circular form of ICESluvan in E. faecium MM5-F9a, E. faecalis OG5-F9a, E. faecalis JH5-F9a, and S. lutetiensis 5-F9.