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. 2013 Dec;195(23):5381–5390. doi: 10.1128/JB.02165-12

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Fig 3 — PFGE of SmaI-digested total DNA of recipient, donor, and transconjugants from filter matings. Lanes 1 and 9, low-range PFGE marker; lane 2, ICESluvan donor S. lutetiensis 5-F9; lane 3, recipient BM4110 containing pIP964; lane 4, 1st-generation transconjugant/2nd-generation donor BM4110 ICESluvan pIP964; lane 5, 2nd-generation transconjugant JH2-2 ICESluvan pIP964; lane 6, 2nd-generation transconjugant UV202 ICESluvan pIP964; lane 7, recipient JH2-2; lane 8, recipient UV202. Vancomycin, streptomycin, and rifampin and fusidic acid resistance are indicated by plus signs opposite Van^r, Str^r, and Rif^r Fus^r, respectively. The presence of ICESluvan was verified by vanB PCR and visual inspection of the SmaI PFGE pattern, where ICESluvan insertion in a 240-kb band results in replacement of one of the 240-kb double bands with a 340-kb band, as shown previously for JH2-derived recipients (20). The presence of the pheromone-responsive plasmid pIP964 was verified by rep_pCF10 and agg PCRs.