Fig 4.

(A) Operon into which ICESluvan has been integrated in E. faecium MM5-F9a compared to the corresponding region in the fully sequenced E. faecalis OG1-RF (accession number CP002621). Dark gray shading indicates regions of similarity between compared CDSs. The tRNA (uracil-5)-methyltransferase rumA disrupted by the insertion is colored light gray, and the insertion is indicated with a dotted line. (B) Sequence comparison of the insertion regions of ICESluvan in E. faecium MM5-F9a and E. faecalis OG5-F9a and the corresponding regions in their recipient strains, E. faecium BM4105-RF and E. faecalis OG1-RF, respectively. Vertical lines indicate identical nucleotides. Left- and right-end sequences of ICESluvan are shown in uppercase. Amino acid residues of CDSs that have been disrupted upon insertion of ICESluvan are marked (capital and boldface), together with their respective codons. #, stop codon.