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. 2013 Dec;195(23):5262–5272. doi: 10.1128/JB.00697-13

Fig 3.

Fig 3

SecDF promotes LLO, PlcA, PlcB, and ActA membrane translocation. (A) Growth curves of WT L. monocytogenes and the ΔsecDF mutant in LB-Glu-1P media. Data are representative of 5 biological experiments (n = 5). (B) Western blot analysis of LLO preproteins' subcellular distribution within the membrane, cytosol, and supernatant fractions of WT L. monocytogenes and the ΔsecDF mutant grown in LB-Glu-1P media using anti-LLO antibodies. GroEL was used as a control protein. The data are representative of three biological repeats (n = 3). (C) Western blot analysis of PlcA and PlcB preproteins' subcellular distribution in the membrane, cytosol, and supernatant fractions of WT L. monocytogenes and ΔsecDF mutant grown in LB-Glu-1P media using specific antibodies. GroEL was used as a control protein. The data are representative of three biological repeats (n = 3). (D) Western blot analysis of 6His-tagged ActA preprotein subcellular distribution in the membrane, cytosol, and supernatant fractions of WT L. monocytogenes and the ΔsecDF mutant grown in LB-Glu-1P media using anti-His tag antibodies. GroEL was used as a control protein. The data are representative of three biological repeats (n = 3).