Table 2.
List of strains and mutants used in this study
| Name | LMRG gene/L. monocytogenes EGDe gene identifier | Description and/or source |
|---|---|---|
| WT | L. monocytogenes 10403S, parental strain used in this study; Daniel A. Portnoy, laboratory stock | |
| Δhly | LMRG_02076/lmo0202 | Deletion of hly gene encoding LLO DP-L2161; 6 |
| ΔplcAB | LMRG_02627/lmo0205 | Deletion of plcA and plcB genes |
| LMRG_02623/lmo0201 | DP-L1936; 8 | |
| ΔsecDF | LMRG_01443/lmo1527 | Deletion of secDF gene, AH-L101; this study |
| ΔsecDF + pLIV2-secDF | ΔsecDF mutant harboring the integrative plasmid pLIV2 encoding the secDF gene under inducible promoter, AH-L102; this study | |
| ΔyidC1 | LMRG_00831/lmo1379 | Deletion of yidC1 gene, AH-L103; this study |
| ΔsecA2 | LMRG_00265/lmo0583 | Deletion of secA2 gene, DP-L4342; 24 |
| WT actA-6his-tag | LMRG_02626/lmo0204 | L. monocytogenes 10403S, encoding 6 histidines at the 3′ end of actA gene (chromosomally), AH-L104; this study |
| WT + pPL2-mCherry | L. monocytogenes 10403S harboring the integrative plasmid pPL2 carrying mCherry gene under a constitutive promoter; this study | |
| ΔsecDF + pPL2-mCherry | ΔsecDF mutant harboring the integrative plasmid pPL2 carrying mCherry gene under a constitutive promoter; this study |