Fig 1.

Sensitivity of nongrowing H. pylori wild-type and Δmsr mutant cells to HOCl. H. pylori SS1 (wild-type) and SS1 Δmsr mutant cells were grown on BA plates, harvested, and resuspended in PBS containing the indicated HOCl concentration for 30 min at room temperature. Cells were serially diluted and plated on BA plates, and CFU were counted after 3 to 5 days. Data shown represent the averages and standard deviations of the results of quadruplicate assays from one experiment, and the mutant is significantly more sensitive than the parent at 125 and 250 μM HOCl (P < 0.05, as determined by Student's t test). *, no CFU could be detected (detection limit, 100 CFU/ml).