Fig 2.

Identification and quantification of HOCl-oxidized and Msr-repaired methionine residues in H. pylori AhpC by LC-MS/MS. AhpC was oxidized with a 60-fold molar excess of HOCl and subsequently repaired (or not) by Msr. Repaired and unrepaired AhpC samples were subjected to in-solution GluC digestion, and Met residues were analyzed and quantified by LC-MS/MS. The values corresponding to the percentages of oxidation (y axis) correspond to the ratios of MetO residues to the total number of times the peptide was found (Met coverage). Error bars represent 1 standard deviation (from three measurements). *, the unoxidized peptide was below our limit of detection.