Fig 3.
SDS-PAGE of native and variant AhpC proteins. Oxidized native (wild-type) AhpC (WTox), nonoxidized native AhpC (WT), or variant AhpC (M101I, M133I) (2.5 μg per lane) proteins were mixed with reducing buffer and heated at 95°C for 5 min or mixed with nonreducing loading buffer (and not heated) before being separated on an SDS-12.5% polyacrylamide gel. A molecular mass standard (M) is shown on the right, with sizes indicated on the right. Calculated (theoretical) molecular mass of AhpC-His6, 23.3 kDa.