Fig 6.

(Left) SDS-PAGE (12%) of supernatants from cultures of the wild type (odd-numbered lanes) and the Δsll1951 strain (even-numbered lanes) that were harvested during exponential growth (OD₇₃₀, ∼0.6), after 2-h treatments with 0.5% N-lauryl sarcosine (lanes 3 and 4) or 0.5% Triton X-100 (lanes 5 and 6). Sll1951 was present in the wild-type samples, as indicated by the arrows. Untreated controls are shown in lanes 1 and 2. (Middle) SDS-PAGE (10%) of supernatants of Δsll1951 (odd-numbered lanes) and wild-type (even-numbered lanes) cultures after treatment with 6 M urea–5 mM EDTA (lanes 1 and 2) or 5 mM EDTA at (lanes 3 and 4) at 60°C for 1 h. Empty lanes between lanes 1 and 2 and between 2 and 3 were cropped. (Right) Glycosylation stain of SDS-PAGE (10%) of supernatants of wild-type (lane 1) and Δsll1951 (lane 2) cultures after treatment with 6 M urea–5 mM EDTA for 1 h. Lanes between lanes 1 and 2 and between 2 and the marker (M) were cropped.