Fig 7.

SDS-PAGE gradient gel (12 to 20%) of supernatants of wild-type (lanes 1 to 3) and the Δsll1951 (lanes 4 to 6) cultures that were grown to high density (OD₇₃₀, >2). Immediately after harvesting, the cell pellets were treated with either 0.5% N-lauryl sarcosine (lanes 2 and 5) or 0.5% Triton X-100 (lanes 3 and 6) for 2 h and were centrifuged again, resulting in the supernatant that was run on the gel. Untreated controls were loaded onto lanes 1 and 4. The panel to the right is a magnification of the area in the left panel outlined by a rectangle. Protein bands were isolated from the gel at the positions indicated by arrows and letters and subjected to mass spectrometry as indicated in Table 2.