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. 2013 Dec;81(12):4498–4508. doi: 10.1128/IAI.00818-13

Fig 3.

Fig 3

Confocal microscopy showing association of wild-type encapsulated S. suis serotype 2 strain 31533 and its nonencapsulated mutant (CPS−) with virus-free cells (control) or swH1N1-preinfected NPTr cells. Cells were noninfected (control) or virus infected for 12 h with swH1N1 (MOI, 1) and then infected with either the S. suis wild-type strain or the CPS− mutant strain (MOI, 10) for 2 h. Samples were labeled using polyclonal antibodies conjugated with Alexa Fluor 488 against S. suis (green) and a mouse monoclonal antibody against influenza virus A nucleoprotein H1N1 conjugated with Alexa Fluor 568 against swH1N1 (red). (A) Wild-type S. suis strain 31533 shows a high level of interactions with cells only when they are preinfected with swH1N1. Nonencapsulated S. suis-cell interaction is not altered by preinfection with influenza virus. DIC, differential interference contrast. (B) High level of adhesion to/invasion of swH1N1-preinfected cells by S. suis strain 31533. Scale bar, 10 μm. Original magnification, ×100.