Fig 7.

Gene expression of proinflammatory mediators by NPTr cells. NPTr cells were either preinfected with swH1N1 for 12 h (MOI, 1) or left untreated, and subsequently the cells were infected with S. suis serotype 2 strain 31533 (MOI, 10) for 24 h. Total RNA was extracted from S. suis- and virus-coinfected cells (V+B), virus-infected cells (V), or bacterium-infected cells (B), and quantitative PCR analysis of selected genes was performed. Normalization of the data was done using the reference genes Hprt1 and Ppia. Mock noninfected samples were used as the calibrator, and relative fold differences were calculated for the rest of the samples and compared to the means from the calibrator samples. Data represent mean values ± standard errors of the means from relative fold expression. Groups that are significantly different from each other are indicated by different letters (a, b, and c), as determined by one-way ANOVA with P ≤ 0.01.