Table 2.
Sequences of porcine-specific real-time PCR primers
| Gene | GenBank accession no. | Amplicon size (bp) | Sequence |
% efficiency (qPCR) | |
|---|---|---|---|---|---|
| Forward | Reverse | ||||
| Hprt1 | NM_001032376 | 142 | GCAGCCCCAGCGTCGTGATT | CGAGCAAGCCGTTCAGTCCTGT | 99 |
| Ppia | NM_214353 | 133 | TGCAGACAAAGTTCCAAAGACAG | GCCACCAGTGCCATTATGG | 97 |
| Ccl2 | NM_214214 | 169 | CAGGTCCTTGCCCAGCCAGATG | CACAGATCTCCTTGCCCGCGA | 90 |
| Ccl4 | NM_213779 | 125 | TCCCACCTCCTGCTGCTTCACAT | GCCTGCCCTTTTTGGTCTGGAA | 100 |
| Il6 | NM_214399 | 105 | ACTCCCTCTCCACAAGCGCCTT | TGGCATCTTCTTCCAGGCGTCCC | 97 |
| Il8 | NM_003300390 | 80 | TGTGAGGCTGCAGTTCTGGCAAG | GGGTGGAAAGGTGTGGAATGCGT | 95 |
| Ifnβ | NM_001003923.1 | 150 | TGCAACCACCACAATTCCAGAAGG | TCTGCCCATCAAGTTCCACAAGGA | 96 |
| Tnf | NM_214022 | 112 | GCCACCACGCTCTTCTGCCTA | ACGATGATCTGAGTCCTTGGGCCA | 91 |