Fig 3.

Humoral immune responses in Bp82-vaccinated mice. Serum was prepared from BALB/c and C57BL/6 mice (n = 5) vaccinated twice s.c. with Bp82, and titers of antibodies to heat-killed Bp82 were determined by endpoint dilution ELISA. Titers of total IgG (A), IgM (B), IgG1 (C), and IgG2a (D) were expressed as the reciprocal of the endpoint dilution. Significant differences (*, P < 0.05; **, P < 0.005; ***, P < 0.0001) were determined by a nonparametric Kruskal-Wallis test followed by Dunnett's multiple comparison test. Data represent pooled data from two independent experiments.