Fig 4.
Cellular immune responses to Bp82 immunization. BALB/c mice (n = 5) were immunized twice s.c. with Bp82. Fourteen days after the second immunization, spleen cells from naive or vaccinated animals were restimulated in vitro with heat-killed Bp82 (2 × 107 CFU/ml), lysate from Bp82 (5 μg/ml), recombinant GroEL (12.5 μg/ml), or medium alone for 3 days. Supernatants were assayed by ELISA for IFN-γ (A) and IL-17 (B) production. *, P < 0.05 using a Mann-Whitney U test. Data represent pooled data from two independent experiments.