Table 2.

Cloning and PCR primers used in this study

Primer	Sequence (5′→3′)a	Use (bp location)b
Ssa.dsd1seq/BamHI	CACGGATCCGAAATTGCTGAAGATGCG	Cloning
Ssa.dsd1rev/HindIII	GTGAAGCTTTGTACGATGATCGTAAGG	Cloning
Ssa.dsd2seq/PstI	CAGCTGCAGTTTACTTTAGTTCGGTAGAG	Cloning
Ssa.dsd2/2rev/XbaI	GTGTCTAGAGTTCCTGACTCTTTGTGG	Cloning
Ssa.dsd4rev/HindIII	GCGAAGCTTCTATAAGCAAGATTTACC	Cloning
Ssa.dsd4seq/BamHI	GTAGGATCCAACGATTTAGCAACACTT	Cloning
Ssa.dsd10rev	TCACCTCGCTATATCTGG	PCR
Ssa.dsd10seq	TTTCTTCCACTGACGTGC	PCR
catseq	GTTACAGTAATATTGACT	PCR, DIG probe synthesis (183–201)
catrev	CATAAACAATCCTGCATG	PCR, DIG probe synthesis (872–890)
ermB	TCTAGAACTAGTGGATCCC	PCR, DIG probe synthesis
ermB	TATTGTCTGCAGccgagagtgattggtctt	PCR, DIG probe synthesis
CAT473seq	CAGCAAACTACGTATAGC	PCR, DIG probe synthesis
CAT473rev	CAAGGAATCATTGAAATCG	PCR, DIG probe synthesis
pPS44rev/SstI	ATAGAGCTGGTGACCACTTGTGATAACG	PCR, cloning
pPS44seq/XbaI	TGCTCTAGAACTCGTGTGCATAATTCACGC	PCR, cloning

^aRestriction sites in the 5′ extensions of primers are underlined. Lowercase letters indicate nonhomologous sequences with added restriction sites.

^bDIG, digoxigenin.