Fig 9.

The conductance of a single CyaA pore is affected by N-terminal deletion. Single-pore conductance of CyaA (0.5 nM) (A) or CyaA-ΔN489 (0.01 nM) (B) was determined in 1 M KCl, 10 mM Tris, and 2 mM CaCl₂ (pH 7.4) at membrane potentials of between 50 and 75 mV; the temperature was 25°C. The conductance histograms of at least 500 pore openings were fitted with the sum of Gaussian functions.