Table 2.
Proteins identified in the culture supernatant of E. rhusiopathiae Fujisawa
| Spot no. | N-terminal amino acid sequence | Locus tag | Identical or homologous protein | pI/molecular mass (kDa) |
|---|---|---|---|---|
| 1 | AKDVR | ERH_1267 | Chaperonin GroEL | 4.56/58.4 |
| 2 | ALVSA | ERH_1632 | Fructose-1,6-bisphosphate aldolase, class II (FBA) | 4.73/30.3 |
| 4 | MRKPI | ERH_1335 | Triosephosphate isomerase (TPI) | 4.59/26.9 |
| 5 | MNLLI | ERH_1152 | Adenylate kinases (AK) | 4.70/24.4 |
| 8 | MKVLI | ERH_0382 | Type I pyroglutamyl-peptidase (PGP) | 5.00/23.1 |
| 11 | TVKVA | ERH_1534 | Glyceraldehyde-3-phosphate dehydrogenase, type I (GAPDH) | 5.32/35.5 |
| 12 | TVKVA | ERH_1534 | Glyceraldehyde-3-phosphate dehydrogenase, type I (GAPDH) | 5.32/35.5 |
| 13 | MMNGK | ERH_1297 | Inosine-5′-monophosphate dehydrogenase (IMPDH) | 5.52/39.5 |
| 14 | AQVKK | ERH_0438 | Pyruvate dehydrogenase complex E1 component, α-subunit (PDHα) | 5.34/41.6 |
| 24 | MNLKE | ERH_0207 | Diaminopimelate decarboxylase (LysA) | 5.87/44.5a |
| 25 | ALVSA | ERH_1632 | Fructose-1,6-bisphosphate aldolase, class II (FBA) | 4.73/30.3 |
| 31 | DANVD | ERH_1662 | 3D domain-containing hypothetical protein | 4.49/28.7a |
| 32 | MQSII | ERH_0644 | Ribosome recycling factor | 4.68/20.2 |
| 33 | LTLRA | ERH_0928 | Hypothetical protein | 5.12b/19.6 |
a
The molecular mass of the protein observed in the 2-D gel was lower than the predicted value shown in the table.
b
The isoelectric point of the protein observed in the 2-D gel was higher than the predicted value shown in the table.