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. 2013 Dec;33(23):4628–4640. doi: 10.1128/MCB.01590-12

Fig 1.

Fig 1

Murine Bob1 is an instable protein. (A) Schematic representation of the expression construct used to determine relative protein stability. (B) Transient expression of GFP-IRES-tdTomato compared to that of GFP-mBob1-IRES-tdTomato in WEHI-231 cells analyzed at 6 and 24 h after transfection. Histograms represent the ratio of GFP to Tomato for each cell. (C) Fusions of mBob1 to the C terminus and N terminus of GFP relative to GFP alone. (D) Comparison of mBob1 stability with that of selected proteins cloned into the GFP fusion vector. Flow cytometric analysis of WEHI-231 cells evaluated the stability of the following proteins at 16 h posttransfection: murine octamer-binding proteins 1 (Oct 1) and 2 (Oct 2), murine B lymphocyte-induced maturation protein 1/Prdm1 (Blimp 1), murine early B cell factor 1 (Ebf1), murine immunoglobulin enhancer-binding factor E2a/Tcf3 (E47), human paired box protein Pax5 (Pax5), murine hairy and enhancer of split 3 (Hes3), and murine spleen tyrosine kinase (Syk).