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. 2013 Dec;33(23):4628–4640. doi: 10.1128/MCB.01590-12

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Fig 5 — Bob1 ortholog and chimera transcriptional activity. (A) Renilla-normalized luciferase activity of Bob1-containing fusion constructs in the murine fibroblast line Ltk. Photinus luciferase production is driven by the murine immunoglobulin light-chain V_K21.7 promoter. From left to right: GFP only (GFP), GFP-human Bob1 (GhsB), GFP-rabbit Bob1 (GrbB), GFP-chicken Bob1 (GggB), GFP-Xenopus Bob1 (GxlB), GFP-zebrafish Bob1 (GzfB), GFP-catfish Bob1 (GcfB), GFP-mBob1 (GmmB), GFP-cf^1-83m^86-256Bob1 (where cf^1-83 is amino acids 1 to 83 of cfBob1 and m^86-256 is amino acids 86 to 256 of mBob1) (cf mBob1), GFP-m^1-151cf^152-245Bob1 (mBob1 cf), GFP-m^1-85cf^84-151m^152-256Bob1 (mB cf mB), GFP-cf^1-83m^86-151cf^152-245Bob1 (cf mB cf), and a GFP-mBob1 DNA-binding mutant (GmBdbm). (B) Renilla-normalized luciferase activity of Bob1 fusion constructs in (from left to right) WEHI-231, 1B10, and HEK293 cells. Photinus luciferase production is driven by an artificial promoter containing 3 octamer motifs and a TATA box.