Fig 2.

RPL11 or RPL5 depletion represses cell proliferation without inducing cell cycle arrest. (A) Proliferation assay of MRC5 cells transfected with the indicated siRNAs and harvested and counted at the indicated time points following transfection. The calculated population doubling times were 34 h, 43 h, and 103 h, respectively, for RPL11-, RPL5-, and RPL7a-depleted cells compared to 20 h for the NS siRNA-treated control cells. (B) Representative cell cycle distribution of the MRC5 cells used in the proliferation assay in panel A at the 24-h time point. Each channel represents the signal intensity of DNA content after amplification. (C) Quantification of the cell cycle distribution of MRC5 cells counted in the proliferation assay in panel A. (D) Representative annexin V/7-AAD staining images of MRC5 cells transfected with indicated siRNAs for 96 h. (E) Quantification of the percentage of annexin V and 7-AAD staining of the samples from panel D. Each bar in panels A, C, and E represents the means ± standard errors of the means from three independent experiments. Statistical significance was determined using a two-tailed t test. ns, not significant.