Fig 5.

Delay of entry into S phase and of accumulation of key cyclins and associated kinase activity in RPL11-depleted cells. MRC5 cells transfected with the indicated siRNAs were synchronized in G₀/G₁ by serum starvation and harvested at the indicated time points following serum stimulation. Parallel samples were processed for cell cycle analysis by flow cytometry (A and B), Western blot analysis of cyclin E and cyclin A protein expression (C), and measurement of cyclin E-associated kinase activity by in vitro kinase assay (D). IP, immunoprecipitation; p-histone H1, phospho-histone H1; siNS, NS siRNA; siL11, siRNA targeting RPL11.