Fig 5.

Depletion of Alk3 in dental epithelial upregulated activity of Wnt/β-catenin signaling but not that of TGF-β signaling. (A to C) AKO and WT DEpSCs were induced as described in the legend to Fig. 4 and analyzed by Western blotting of nuclear proteins (A), qRT-PCR (B), or IF (C) to examine the expression of genes related to the Wnt/β-catenin pathway or the TGF-β pathway (n = 3 in each assay) (n.s., not significant; ∗, P < 0.05). (D) P7 WT and AKO teeth were examined with double IF for β-catenin and Krt14.