Table 1.

Characterization of samples, based on conventional antibiotic susceptibility, mutation(s) in PBP 2, and NG-MAST^a

Patient no.	Sample type	Susceptibility (MIC, in μg/ml)b						penA mutationc			Allele no.d		STe
		Penicillin	Tetracycline	Ceftriaxone	Cefixime	Ciprofloxacin	Azithromycin	I312M	V316T	G545S	por	tbpB
1	Urethra	2	1	0.06	0.12	>32	0.5	M	T	S	908	110	1407
2	Urethra	2	2	0.06	0.12	≥32	0.5	M	T	S	908	110	1407
3	Urethra	2	0.5	0.06	0.12	≥32	0.5	M	T	S	3669	110	6200
4	Cervix	2	0.5	0.06	0.12	>32	0.5	M	T	S	2743	110	4461
5	Urethra	2	1	0.06	0.12	16	0.5	M	T	S	1914	110	3158
6	Urethra	2	1	0.06	0.12	≥32	0.5	M	T	S	908	110	1407
7	Urethra	0.25	0.25	0.016	0.06	4	0.06	M	T	S	908	110	1407
8	Cervix	0.25	0.12	0.25	≤0.016	≥32	0.25	I	V	G	1910	27	4637
9	Urethra	0.5	1	0.016	≤0.016	8	0.25	I	V	G	1489	563	2400
10	Urethra	0.25	0.5	0.008	≤0.016	0.008	1	I	V	G	5108	29	8632
11	Urethra	0.25	0.5	0.016	≤0.016	0.008	0.5	I	V	G	908	29	3935
12	Urethra	0.5	1	0.016	≤0.016	0.008	1	I	V	G	908	29	3935
13	Urethra	0.25	8	0.004	≤0.016	≤0.002	0.06	I	V	G	2649	1066	5083
14	Urethra	0.12	0.5	0.008	≤0.016	≤0.002	0.25	I	V	G	1808	29	2992
15	Urethra	0.5	0.5	0.012	≤0.016	0.008	1	I	V	G	908	29	3935
16	Urethra	0.25	0.5	0.008	≤0.016	0.016	1	I	V	G	908	29	3935
17	Urethra	1	0.25	0.008	≤0.016	4	0.25	I	V	G	2852	25	4709
18	Cervix	1	0.25	0.008	≤0.016	4	0.25	I	V	G	2852	25	4709
19	Urethra	1	0.5	0.016	≤0.016	4	0.5	I	V	G	2852	25	4709
20	Urethra	1	1	0.016	≤0.016	4	0.5	I	V	G	53	10	657
21	Urethra	0.5	1	0.008	≤0.016	0.008	1	I	V	G	908	29	3935
22	Cervix	0.5	0.5	0.016	≤0.016	0.016	1	I	V	G	908	29	3935
23	Urethra	0.12	0.12	0.004	≤0.016	0.002	0.12	I	V	G	2155	16	3556
24	Urethra	0.12	0.25	0.004	≤0.016	0.004	0.125	I	V	G	852	19	1319
25	Urethra	0.25	0.5	0.008	≤0.016	0.008	0.25	I	V	G	1808	29	2992
26	Cervix	0.25	0.5	0.008	≤0.016	0.008	1	I	V	G	908	29	3935
27	Urethra	0.25	0.25	0.008	≤0.016	0.002	0.5	I	V	G	1808	29	2992
28	Urethra	0.03	0.12	≤0.016	≤0.016	≤0.016	0.12	I	V	G	55	4	69
29	Urethra	0.25	0.25	0.008	≤0.016	0.004	0.5	I	V	G	1808	29	2992
30	Urethra	1	1	0.03	0.03	>32	0.12	I	V	G	908	563	7574

^aSamples with ESC^ds phenotype, related allele numbers, and sequence types are shown in bold.

^bAntibiotic susceptibility profiles were determined with Etest strips.

^cAmino acid alteration(s) in the penA gene (I312M, V316T, and G545S).

^dAllele number, based on the por and tbpB genes.

^eST, based on NG-MAST result.