Fig 3.
Distribution of real-time PCR threshold cycle (CT) values for cDNA samples from cell culture supernatants of human rhinovirus (HRV) and human enterovirus (HEV) prototype strains and clinical specimens. Scatter plots of CT values observed with virus-specific probes over dsDNA dye BOXTO are shown. (A) HRV prototype strains (types 1 to 100, including 75 HRV-A and 25 HRV-B strains) detected with FAM-RIp. All strains were negative with the HEV-specific probe. (B) HEV prototype strains (12 HEV-A, 35 HEV-B, 15 HEV-C, and 4 HEV-D strains) detected with Cy5-ENp. CVA1 (black circle; value on the x axis for visualization only) was detected with BOXTO alone. All strains were negative with the HRV-specific probe. (C) Clinical specimens with HRVs (n = 58). (D) Clinical specimens with HEVs (n = 58). A conjunctival fluid specimen with CVA24 (black circle) was detected by ENp only. Clinical specimens that were tested after culture are shown as gray circles. In each plot, the dotted line represents a linear regression line with the indicated equation and coefficient of determination, R2.