Table 3.

Standard curve parameters and amplification efficiencies for different plasmid virus cDNAs in PCR^a

Plasmid virus	Intercept parameters with:			Slope parameters with:			Efficiency parameters with:
	FAM-RIp	Cy5-ENp	BOXTO	FAM-RIp	Cy5-ENp	BOXTO	FAM-RIp	Cy5-ENp	BOXTO
HRV-A1b	40.17		39.15	−3.43		−3.31	0.96		1.00
HRV-B14	38.92		41.25	−3.48		−3.35	0.94		0.99
HRV-A85	40.04		41.93	−3.37		−3.30	0.98		1.01
CVA16		39.97	39.82		−3.36	−3.32		0.98	1.00
CVB4		40.71	39.20		−3.33	−3.24		1.00	1.03
EV11		39.46	42.60		−3.33	−3.24		1.00	1.04

^aLinear regression curves (y = mx + b, where y = C_T, m = slope, x = log₁₀[copies/PCR], and b = intercept when x = 0) and efficiencies (E = 10^−1/m − 1) were determined with 10-fold dilutions containing 5 × 10⁷ to 5 plasmid copies/reaction.