Table 1.
ProGastro SSCS targets and sequencing primers used for discrepant analysis
| Genus or gene target | Primer direction and sequence (5′ to 3′) | Sequencing target amplicon size (bp) | Sequencing assay gene target | ProGastro SSCS gene target |
|---|---|---|---|---|
| Salmonella | Forward, CCTGTCAGCCAAATATTACG | 344 | ttRSBCA | orgC |
| Reverse, ACGACGGGTTAAATTAGCCA | ||||
| Shigella | Forward, TGAAGTTTCTCTGCGAGCAT | 342 | ipaH | ipaH |
| Reverse, CAATACCTCCGGATTCCG | ||||
| Campylobacter | Forward, GGATGACACTTTTCGGAGC | 820 | 16S rRNA | glyA (C. jejuni) |
| Reverse, CATTGTAGCACGTGTGTC | cadF (C. coli) | |||
| stx1 | Forward, TGACAGTAGCTATACCACGT | 329 | stx1 | stx1 |
| Reverse, GAACAGAGTCTTGTCCATGA | ||||
| stx2 | Forward, GGACCTCACTCTGAACTG | 376 | stx2 | stx2 |
| Reverse, CCGCCATTGCATTAACAGAA |