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Cq values generated by assaying amphibian samples with the Batrachochytrium dendrobatidis and B. salamandrivorans duplex real-time PCR assay and both simplex real-time PCR assays. An overview of all assayed amphibian samples is presented in Table 1. Samples that did not generate a signal were assigned a Cq value of 40, corresponding to the maximum number of cycles run in this real-time PCR setup.
