Table 1.

Overview of the Chytridiomycota isolates used to verify the specificity of the real-time duplex PCR for B. dendrobatidis and B. salamandrivorans

Species	Class	Order	Isolate	Amplificationa
Batrachochytrium dendrobatidis	Chytridiomycetes	Rhizophydiales	JEL423	Yes (B. dendrobatidis)
Batrachochytrium salamandrivorans	Chytridiomycetes	Rhizophydiales	AMFP13/1	Yes (B. salamandrivorans)
Homolaphlyctis polyrhiza	Chytridiomycetes	Rhizophydiales	JEL142	No
Rhizophlyctis rosea	Chytridiomycetes	Rhizophlyctidales	JEL532	No
Gaertneriomyces semiglobifer	Chytridiomycetes	Spizellomycetales	JEL384	No
Rhizoclosmatium globosum	Chytridiomycetes	Chytridiales	JEL791	No
Podochytrium dentatum	Chytridiomycetes	Chytridiales	JEL30	No
Polychytrium aggregatum	Chytridiomycetes	Polychytriales	JEL109	No
Geranomyces variabilis	Chytridiomycetes	Spizellomycetales	JEL518	No
Monoblepharis polymorpha	Monoblepharidomycetes	Monoblepharidales	JEL486	No

^aThe component of the duplex real-time PCR that showed amplification in positive samples is indicated in parentheses.