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Assay precision, efficiencies, and linear correlations of the B. dendrobatidis and B. salamandrivorans real-time duplex PCRs
| Species and variability experiment | Efficiency (%) | Linear correlation (r2) | Coefficient of variation (% [mean ± SD]) |
|---|---|---|---|
| B. dendrobatidis | |||
| Intra-assay | 94.1 | 0.997 | 0.56 ± 0.34 |
| Interassay | 99.4 | 0.996 | 0.99 ± 0.39 |
| B. salamandrivorans | |||
| Intra-assay | 95.7 | 0.999 | 0.39 ± 0.48 |
| Interassay | 96.0 | 0.997 | 0.98 ± 0.27 |
