Table 4.
Overview of the amphibian samples used to validate the real-time duplex PCRs for B. dendrobatidis and B. salamandrivorans
| Amphibian species | Origin (coordinates and/or reference) and yr | Health status | Skin sample type (n) | No. PCR positive (species) | Mean GEs/swab for positive samples (range)a |
|---|---|---|---|---|---|
| Fire salamander (Salamandra salamandra) | Bunderbos, Netherlands (N50°55′, E5°45′), 2010 | Declining | Swab (33) | 13 (B. salamandrivorans) | 219.8 (60–1,750) |
| Tissue (8) | 4 (B. salamandrivorans) | 2,398.3 (242–10,180) | |||
| Merelbeke, Belgium (N50°57′, E3°43′), 2012 | Healthy | Swab (51) | 0 | NA | |
| B. salamandrivorans infection experiment (6), 2013 | Diseased | Swab (5) | 5 (B. salamandrivorans) | 6,920 (1,572–10,740) | |
| Tissue (5) | 5 (B. salamandrivorans) | 10,915 (3,420–19,380) | |||
| B. salamandrivorans infection experiment (6), 2013 | Healthy | Swab (5) | 0 | NA | |
| Tissue (5) | 0 | NA | |||
| Yellow-bellied toad (Bombina variegata) | ‘t Rooth, Netherlands (N50°50′, E5°47′) (A. Spitzen-van der Sluijs, A. Martel, C. A. Hallmann, W. Bosman, T. W. J. Garner, P. van Rooij, R. Jooris, F. Haesebrouck, and F. Pasmans, submitted for publication), 2013 | Healthy | Swab (27) | 14 (B. dendrobatidis) | 175.4 (20–1,488) |
a
Mean values of genomic equivalents (GEs) of zoospores per swab of B. dendrobatidis and B. salamandrivorans for the positive samples were calculated with the included quantitation standards for B. dendrobatidis and B. salamandrivorans. NA, not applicable.