Table 1.
Identification results of yeast isolates by Biotyper and Vitek MS IVD (n = 98)
| Yeast/fungal species | No. of isolates | MALDI-TOF MS results (no. [%]) usinga: |
|||||
|---|---|---|---|---|---|---|---|
| Biotyper plus fungus RUO database |
Vitek MS IVD |
||||||
| Unreliable IDb (score, <1.6) | Genus-level ID only (score, 1.6–1.99) | Species-level ID (≥2.0) | Unreliable IDb (<90.0%) | Correct ID (90.0–97.9%) | Correct ID (≥98.0%) | ||
| Candida spp. | |||||||
| C. albicans | 24 | 0 (0) | 2 (8.3) | 22 (91.7) | 1 (4.2) | 0 (0) | 23 (95.8) |
| C. boidinii | 1 | 1 (100) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 1 (100) |
| C. dubliniensis | 3 | 0 (0) | 1 (33.3) | 2 (66.7) | 3 (100) | 0 (0) | 0 (0) |
| C. glabrata | 5 | 0 (0) | 0 (0) | 5 (100) | 0 (0) | 1 (20.0) | 4 (80.0) |
| C. guilliermondii | 2 | 0 (0) | 1 (50.0) | 1 (50.0) | 0 (0) | 0 (0) | 2 (100) |
| C. kefyr (Kluyveromyces marxianus) | 3 | 0 (0) | 0 (0) | 3 (100) | 0 (0) | 0 (0) | 3 (100) |
| C. krusei (Issatchenkia orientalis) | 3 | 0 (0) | 0 (0) | 3 (100) | 0 (0) | 0 (0) | 3 (100) |
| C. lipolytica | 2 | 0 (0) | 1 (50.0) | 1 (50.0) | 0 (0) | 0 (0) | 2 (100) |
| C. magnoliae | 1 | 1 (100) | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 1 (100) |
| C. norvegensis | 4 | 0 (0) | 0 (0) | 4 (100) | 0 (0) | 0 (0) | 4 (100) |
| C. parapsilosis | 18 | 0 (0) | 5 (27.8) | 13 (72.2) | 4 (22.2) | 1 (5.6) | 13 (72.2) |
| C. pararugosa | 1 | 0 (0) | 0 (0) | 1 (100) | 1 (100) | 0 (0) | 0 (0) |
| C. rugosa | 2 | 1 (50) | 1 (50) | 0 (0) | 1 (50) | 0 (0) | 1 (50) |
| C. tropicalis | 7 | 0 (0) | 0 (0) | 7 (100) | 0 (0) | 0 (0) | 7 (100) |
| C. (Clavispora) lusitaniae | 2 | 0 (0) | 1 (50) | 1 (50) | 0 (0) | 0 (0) | 2 (100) |
| Subtotal | 78 | 3 (3.8) | 12 (15.4) | 63 (80.8) | 10 (12.8) | 2 (2.6) | 66 (84.6) |
| Other yeast and yeast-like fungi | |||||||
| Blastoschizomyces capitatus | 1 | 0 (0) | 1 (100) | 0 (0) | 0 (0) | 0 (0) | 1 (100) |
| Cryptococcus humicola (Trichosporon mucoides) | 2 | 0 (0) | 1 (50) | 1 (50) | 2 (100) | 0 (0) | 0 (0) |
| Cryptococcus neoformans | 7 | 1 (14.3) | 1 (14.3) | 5 (71.4) | 6 (85.7) | 0 (0) | 1 (14.3) |
| Galactomyces candidum | 1 | 0 (0) | 1 (100) | 0 (0) | 0 (0) | 0 (0) | 1 (100) |
| Pichia anomala (Candida pelliculosa) | 1 | 0 (0) | 0 (0) | 1 (100) | 0 (0) | 0 (0) | 1 (100) |
| Pichia ohmeri | 2 | 0 (0) | 2 (100) | 0 (0) | 2 (100) | 0 (0) | 0 (0) |
| Pseudozyma parantarctica | 1 | 1 (100) | 0 (0) | 0 (0) | 1 (100) | 0 (0) | 0 (0) |
| Saccharomyces cerevisiae | 1 | 0 (0) | 0 (0) | 1 (100) | 0 (0) | 0 (0) | 1 (100) |
| Trichosporon asahii | 2 | 0 (0) | 0 (0) | 2 (100) | 0 (0) | 0 (0) | 2 (100) |
| Trichosporon dermatis | 2 | 0 (0) | 2 (100) | 0 (0) | 2 (100) | 0 (0) | 0 (0) |
| Subtotal | 20 | 2 (10.0) | 8 (40.0) | 10 (50.0) | 13 (65.0) | 0 (0) | 7 (35.0) |
| Total | 98 | 5 (5.1) | 20 (20.4) | 73 (74.5) | 23 (23.5) | 2 (2.0) | 73 (74.5) |
a
RUO, research use only; ID, identification.
b
Includes the no identification and low discrimination results.