Skip to main content
. Author manuscript; available in PMC: 2014 Oct 9.
Published in final edited form as: J Am Chem Soc. 2013 Sep 24;135(40):10.1021/ja4072964. doi: 10.1021/ja4072964

Figure 4.

Figure 4

Representative confocal microscopy images of HepG2/C3A cells stained with 20 μM IP1-AM for 2 hrs. (A) Control cells. (B) Cells treated with 1 mM ascorbic acid (Asb) for 40 min. (C) Cells treated with 1 mM ascorbic acid for 40 min and then 50 μM desferoxamine (DFO) for a further 40 min. (D) Quantification of relative fluorescence intensities of confocal microscopy images of HepG2/C3A cells obtained under conditions A–C. Error bars are ±SD (n = 3). Statistical analysis was performed with a two-tailed Student’s t-test, *: p <0.001. Fluorescent intensity in images A–C is represented in a pseudo-color scale for better visual contrast.