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Binding analysis of Δ2 LHBS with JAB1 in the presence of different metal ions. (A) Surface plasmon resonance analysis to measure the binding affinities of Δ2 LHBS with JAB1 in the presence of 20 μM Zn2+ (A) and Ni2+ ions (B). Δ2 LHBS in the indicated concentrations was injected onto the sensor chip on which JAB1 had been immobilized. Reactions of the protein association and dissociation were monitored for 4 min. RU, response unit.
