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ITC analysis of ZnCl2 binding to Δ2 LHBS and its H71Y/H116Y double mutant. The raw heat change data for each injection from ITC analysis of Zn2+ binding to Δ2 LHBS (A) and Zn2+ binding to the H71Y/H116Y double mutant of Δ2 LHBS (B) are presented.