Table 1.
PCR primers used in this study
| Gene | Primer |
|
|---|---|---|
| Orientationa | Sequence (5′–3′) | |
| H28Y | F | GGGATTCTTTCCCGATTATCAGTTGGACCCTGC |
| R | GCAGGGTCCAACTGATAATCGGGAAAGAATCCC | |
| H71Y | F | CAGGGCTCACCCCTCCATACGGCGCTATTTTGGGG |
| R | CCCCAAAATACCGCCGTATGGAGGGGTGAGCCCTG | |
| H116Y | F | CTCTCCACCTCTAAGAGACAGTTATCCTCAGGCCATACAGTGG |
| R | CCACTGTATGGCCTGAGGATAACTGTCTCTTAGAGGTGGTGGAGAG | |
| JAB1ΔJAMM | F | GCTACAAAAAAGTAATGGTGATCCTTAGTCC |
| R | ATTACTTTTTTGTAGCAGTGGTGATTGATCC | |
| JAB1Δ1-52 | F | GAATTCGCAAGTACTGCAAAATC |
| JAB1Δ251-334 | R | CTCGAGCAACGTATTCACCCAGTA |
| H138Y | F | GCAATCGGGTGGTATTATAGCCACCCTGGCTATG |
| R | CATAGCCAGGGTGGCTATAATACCACCCGATTGC | |
a
F, forward; R, reverse.