Table 2.

Kinetic constants of protein interaction between JAB1 and the full-length and Δ61-119 Δ2 LHBS in the presence of Zn²⁺ and Ni²⁺ ions

Δ2 LHBS	Metal ion (20 μM)	Mean ± SEM
		kon (M−1 s−1)	koff (s−1)	KD (M)
Full length	Ni2+	(5.92 ± 0.14) × 102	(1.08 ± 0.09) × 10−2	(1.82 ± 0.10) × 10−5
	Zn2+	(1.44 ± 0.07) × 105	(1.17 ± 0.03) × 10−2	(8.09 ± 0.60) × 10−8
Δ61-119	Zn2+	(8.32 ± 0.87) × 103	(8.95 ± 0.70) × 10−3	(1.08 ± 0.11) × 10−6