Table 4.
Binding affinities and isothermal properties of Zn2+ ions to Δ2 LHBS and its H71Y/H116Y double mutant (ITC) analysis
| Δ2 LHBS or mutant | Site 1 |
Site 2 |
||
|---|---|---|---|---|
| KD (nM) | ΔH (cal/mol) | KD (nM) | ΔH (cal/mol) | |
| Δ2 LHBS | 81 | –1,555 | 64 | +2,774 |
| Δ2 LHBS/H71Y,H116Y | NDa | ND | 17 | +3,813 |
ND, not determined.