Fig 6.

Semen amyloids bind CMV. (Left panel) A total of 1 ml of HCMV (10⁷/ml) was incubated with SEVI or SEM amyloid fibrils at 50 μg/ml for 1 h at 37°C. Controls include virus alone and virus incubated with 50 μg of Aβ(1-42) amyloids/ml. The mixtures were centrifuged, and the pellet was washed twice with MEM. The pellet was lysed in 2× Laemmli buffer and blotted with anti-IE1 (nonstructural protein), anti-gH (envelope protein), anti-gB (envelope protein), and anti-pp71 (tegument protein) antibodies. Whole-cell lysates (WCL) of HCMV-infected NIH 3T3 cells were used as input controls for the Western blot. (Right panel) Similar conditions, but using MCMV instead of HCMV. The tegument protein detected for MCMV was m25.