Fig 2.

ANDV enhances induction of hypoxia-induced genes. MECs and LECs were ANDV or mock infected and exposed to normoxic or hypoxic conditions for 18 h as in Fig. 1 (47, 49–52). Total cellular RNA was extracted, and human VEGF A, angiopoietin 4 (ANG4), and EGLN3 transcript levels were determined by qRT-PCR of duplicate samples (Table 1) (64, 65). mRNA levels were normalized to GAPDH mRNA levels, and the fold increase in mRNA levels over mock-infected controls was calculated. The means and standard errors from two independent experiments are shown.