Fig 5.

ANDV and hypoxia enhance mTOR-directed S6K phosphorylation. MECs (A) or LECs (B) were infected with ANDV and TULV for 2 days under normoxic conditions and subsequently grown under normoxic or hypoxic conditions as in Fig. 1. Three days postinfection, cells were treated with rapamycin (20 ng/ml) for 1 h or left untreated. Cells were lysed with 1% NP-40, and total protein was analyzed by Western blotting using anti-p70S6-Thr389 rabbit polyclonal antibody (Cell Signaling), anti-β-tubulin monoclonal antibody (Santa Cruz Biotechnology), and antinucleocapsid rabbit serum as previously described (65, 111).